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Image Search Results
Journal: British Journal of Cancer
Article Title: Her-2/ neu- triggered intracellular tyrosine kinase activation: in vivo relevance of ligand-independent activation mechanisms and impact upon the efficacy of trastuzumab-based treatment
doi: 10.1038/sj.bjc.6601160
Figure Lengend Snippet: Immunohistochemical analysis of two (grade 3+) Her-2/ neu overexpressing tumours (upper row) differing with respect to Her-2/ neu phosphorylation status at the tyr1248 residue (lower row): strong (left) and absent Her-2/ neu phosphorylation (right).
Article Snippet: In cases of 2+ immunostaining for Her-2/ neu , oncoprotein gene copy number of the HER-2/ neu (c- erb B-2) gene was determined by dual-colour FISH referring to the numbering of chromosome 17 using the
Techniques: Immunohistochemical staining
Journal: British Journal of Cancer
Article Title: Her-2/ neu- triggered intracellular tyrosine kinase activation: in vivo relevance of ligand-independent activation mechanisms and impact upon the efficacy of trastuzumab-based treatment
doi: 10.1038/sj.bjc.6601160
Figure Lengend Snippet: Western blot analysis demonstrating the phosphorylation state specifity of the PN2A antibody. Specifity of ( A ) anti-Her-2/ neu (p185) antibody and ( B ) anti-phospho (tyr1248) Her-2/ neu antibody (PN2A) is shown by Western blot analysis of whole-cell lysates. SKBR3 cells were treated without (lanes A1 and B8) and with 100 ng ml −1 EGF for 2 (lanes A3 and B6), 4 (lanes A4 and B5), 8 (lanes A5 and B4), 10 (lanes A6 and B3), 30 (lanes A7 and B2) and 60 (lanes A8 and B1) min (kDa, molecular weight in kilodalton, molecular weight markers at 250, 98 and 64 kDa).
Article Snippet: In cases of 2+ immunostaining for Her-2/ neu , oncoprotein gene copy number of the HER-2/ neu (c- erb B-2) gene was determined by dual-colour FISH referring to the numbering of chromosome 17 using the
Techniques: Western Blot, Molecular Weight
Journal: British Journal of Cancer
Article Title: Her-2/ neu- triggered intracellular tyrosine kinase activation: in vivo relevance of ligand-independent activation mechanisms and impact upon the efficacy of trastuzumab-based treatment
doi: 10.1038/sj.bjc.6601160
Figure Lengend Snippet: Serum Her-2/ neu ECD levels are depicted in accordance to Her-2/ neu overexpression (grade 2+ vs 3+) and Her-2/ neu phosphorylation status of tumours (extreme outliers not depicted).
Article Snippet: In cases of 2+ immunostaining for Her-2/ neu , oncoprotein gene copy number of the HER-2/ neu (c- erb B-2) gene was determined by dual-colour FISH referring to the numbering of chromosome 17 using the
Techniques: Over Expression
Journal: British Journal of Cancer
Article Title: Her-2/ neu- triggered intracellular tyrosine kinase activation: in vivo relevance of ligand-independent activation mechanisms and impact upon the efficacy of trastuzumab-based treatment
doi: 10.1038/sj.bjc.6601160
Figure Lengend Snippet: Progression-free survival in patients receiving trastuzumab-based treatment for Her-2/ neu overexpressing metastatic breast cancer in accordance to activation status of Her-2/ neu . Full line: unphosphorylated Her-2/ neu , dotted line: phosphorylated Her-2/ neu (log-rank test P <0.0095).
Article Snippet: In cases of 2+ immunostaining for Her-2/ neu , oncoprotein gene copy number of the HER-2/ neu (c- erb B-2) gene was determined by dual-colour FISH referring to the numbering of chromosome 17 using the
Techniques: Activation Assay